Based on the current situation of outdated teaching content and old-fashioned teaching methods in organic chemistry experiment teaching in some domestic colleges and universities, this paper analyzed various advantages and challenges of introducing MOOC teaching in organic chemistry experiment course. It is pointed out that the mode of combining MOOC with traditional teaching methods not only can improve the quality and efficiency of organic chemistry experiment teaching, but also can motivate students’ interest in learning. Moreover, it is conducive to training innovative talents with solid foundation and rich experience.